Agrobacterium tumefaciens-Mediated Transformation ofValsa mali: An Efficient Tool for Random Insertion Mutagenesis

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Agrobacterium tumefaciens-Mediated Transformation of Valsa mali: An Efficient Tool for Random Insertion Mutagenesis

Valsa mali is a causal agent of apple and pear trees canker disease, which is a destructive disease that causes serious economic losses in eastern Asia, especially in China. The lack of an efficient transformation system for Valsa mali retards its investigation, which poses difficulties to control the disease. In this research, a transformation system for this pathogen was established for the f...

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Agrobacterium tumefaciens was used to transform Aspergillus fumigatus by either random or site-directed integration of transforming DNA (T-DNA). Random mutagenesis via Agrobacterium tumefaciens-mediated transformation (ATMT) was accomplished with T-DNA containing a hygromycin resistance cassette. Cocultivation of A. fumigatus conidia and Agrobacterium (1:10 ratio) for 48 h at 24 degrees C resul...

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Agrobacterium tumefaciens-mediated transformation of yeast.

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Agrobacterium tumefaciens- mediated Transformation of Plant Cells

Agrobacterium tumefaciens is a Gram-negative soil bacterium that causes plant tumours by transferring a portion of DNA from a resident ‘tumour inducing’ (Ti) plasmid into plant cells where it is integrated into a plant chromosome and expressed. This bacteria’s capacity for DNA transfer is the basis of most current plant genetic engineering and make it a model system for the study of pathogenic ...

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ژورنال

عنوان ژورنال: The Scientific World Journal

سال: 2013

ISSN: 1537-744X

DOI: 10.1155/2013/968432